Mouse liver metallothioneins. Purification, molecular weight, amino acid composition, and metal content.

Rabbit testis proacrosin. Purification, molecular weight estimation, and amino acid and carbohydrate composition of the molecule.

Rabbit testis contains a single molecular form of proacrosin which could be extracted at acid pH and purified 145-fold by Sephadex G-100, SE-Sephadex, and concanavalin A-Sepharose affinity column chromatography. The purified proacrosin was judged to be homogeneous on the basis of chromatographic analyses, disc and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, and immunodiffusi...

A corticotrophin-releasing factor: partial purification and amino acid composition.

Proximate Composition, Amino Acid, Mineral, and Heavy Metal Content of Dried Laver

Laver, a red algae belonging to the genus Porphyra, is one of the most widely consumed edible seaweeds. The most popular commercial dried laver species, P. tenera and P. haitanensis, were collected from Korea and China, respectively, and evaluated for proximate composition, amino acids, minerals, trace heavy metals, and color. The moisture and ash contents of P. tenera and P. haitanensis ranged...

The purification and the amino acid content of vasopressin preparations.

Since the discovery (1) of the pressor activity of extracts of the posterior lobe of the pituitary gland, many laboratories have investigated the fractionation of the extracts obtained from this gland. The physiological potency of various preparations, as well as the methods of fractionation employed, have already been reviewed in considerable detail (24). In the present paper we report a new m...

Aminotripeptidase of swine kidney. II. Amino acid composition and molecular weight.

Three different forms of aminotripeptidase have been obtained from swine kidney. As studied in the ultracentrifuge by sedimentation-equilibrium, these forms are: 1. a stable monomer of 71,100 molecular weight that consists of a single polypeptide chain lacking disulfide bridges; 2. a stable dimer that contains two apparently identical subunits of the same size as the monomer; and 2’. an unstabl...